Abstract
Neisseria gonorrhoeae surface protein A (NspA) is a highly conserved gonococcal antigen. To explore the potential of NspA in vaccine development against gonorrhea, BALB/c mice were immunized with pcNspA containing the NspA gene from N. gonorrhoeae strain WHO-A via intramuscular (i.m.) injection, intranasal (i.n.) immunization, or intravaginal (i.vag.) immunization. Following the last DNA immunization, mice were boosted with recombinant NspA (rNspA). Enzyme-linked immunosorbent assays (ELISAs) indicated that all immunized mice generated measurable NspA-specific IgG and IgA in serum and secretory IgA (sIgA) in vaginal wash fluids. The antisera had bactericidal and opsonic activities. These data demonstrated that NspA induced antibodies with antigonococcal activity.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Administration, Intranasal
-
Administration, Intravaginal
-
Animals
-
Antibodies, Bacterial / blood*
-
Antigens, Bacterial / immunology*
-
Bacterial Outer Membrane Proteins / immunology*
-
Bacterial Vaccines / administration & dosage
-
Bacterial Vaccines / immunology*
-
Enzyme-Linked Immunosorbent Assay
-
Female
-
Immunization / methods
-
Immunization, Secondary / methods
-
Immunoglobulin A / blood
-
Immunoglobulin G / blood
-
Injections, Intramuscular
-
Mice
-
Mice, Inbred BALB C
-
Neisseria gonorrhoeae / immunology*
-
Opsonin Proteins / blood*
-
Vaccines, DNA / administration & dosage
-
Vaccines, DNA / immunology*
-
Vagina / immunology
Substances
-
Antibodies, Bacterial
-
Antigens, Bacterial
-
Bacterial Outer Membrane Proteins
-
Bacterial Vaccines
-
Immunoglobulin A
-
Immunoglobulin G
-
NspA protein, Neisseria
-
Opsonin Proteins
-
Vaccines, DNA