Differential activation of cAMP- and cGMP-dependent protein kinases by cyclic purine and pyrimidine nucleotides

Sabine Wolter; Marina Golombek; Roland Seifert

doi:10.1016/j.bbrc.2011.10.093

Differential activation of cAMP- and cGMP-dependent protein kinases by cyclic purine and pyrimidine nucleotides

Biochem Biophys Res Commun. 2011 Dec 2;415(4):563-6. doi: 10.1016/j.bbrc.2011.10.093. Epub 2011 Oct 31.

Authors

Sabine Wolter¹, Marina Golombek, Roland Seifert

Affiliation

¹ Institute of Pharmacology, Medical School of Hannover, Carl-Neuberg-Str. 1, D-30625 Hannover, Germany.

PMID: 22074826
DOI: 10.1016/j.bbrc.2011.10.093

Abstract

The cyclic purine nucleotides cAMP and cGMP are well-characterized second messengers and activators of PKA and PKG, respectively. In contrast, the functions of the cyclic pyrimidine nucleotides cCMP and cUMP are poorly understood. cCMP induces relaxation of smooth muscle via PKGI, and phosphodiesterases differentially hydrolyze cNMPs. Here, we report that cNMPs differentially activate PKA isoforms and PKGIα. The combination of cCMP with cAMP reduced the EC(50) of cAMP for PKA. PKGIα exhibited higher specificity for the cognate cNMP than PKA. Our data support a role of cCMP and cUMP as second messengers.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cyclic AMP / chemistry*
Cyclic AMP-Dependent Protein Kinase RIIalpha Subunit / chemistry*
Cyclic AMP-Dependent Protein Kinase RIalpha Subunit / chemistry*
Cyclic GMP / chemistry*
Cyclic GMP-Dependent Protein Kinase Type I
Cyclic GMP-Dependent Protein Kinases / chemistry*
Enzyme Activation
Nucleotides, Cyclic / chemistry*
Uridine Monophosphate / chemistry*

Substances

Cyclic AMP-Dependent Protein Kinase RIIalpha Subunit
Cyclic AMP-Dependent Protein Kinase RIalpha Subunit
Nucleotides, Cyclic
cyclic 3',5'-uridine monophosphate
Cyclic AMP
Uridine Monophosphate
Cyclic GMP-Dependent Protein Kinase Type I
Cyclic GMP-Dependent Protein Kinases
Cyclic GMP