Isolation and identification of native membrane glycoproteins from living cell by concanavalin A-magnetic particle conjugates

Ganglong Yang; Ting Cui; Qiaoling Chen; Tianran Ma; Zheng Li

doi:10.1016/j.ab.2011.10.033

Isolation and identification of native membrane glycoproteins from living cell by concanavalin A-magnetic particle conjugates

Anal Biochem. 2012 Feb 1;421(1):339-41. doi: 10.1016/j.ab.2011.10.033. Epub 2011 Oct 25.

Authors

Ganglong Yang¹, Ting Cui, Qiaoling Chen, Tianran Ma, Zheng Li

Affiliation

¹ Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, 710069 Xi'an, People's Republic of China.

PMID: 22079135
DOI: 10.1016/j.ab.2011.10.033

Abstract

The discovery, isolation, and subsequent identification of cell membrane glycoproteins involved in the structure and function of the cell surface are becoming more and more important. Here, concanavalin A-magnetic particle conjugates were employed to isolate the special membrane glycoproteins from living HepG-2 cells. The isolated glycoproteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and liquid chromatography-tandem mass spectrometry as well as annotated. A total of 37 membrane glycoproteins were identified, and 25 of them were ascertained to locate in the extracellular region.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, Liquid
Concanavalin A
Electrophoresis, Polyacrylamide Gel
Hep G2 Cells
Humans
Magnetite Nanoparticles
Membrane Glycoproteins / chemistry
Membrane Glycoproteins / isolation & purification*
Molecular Weight
Tandem Mass Spectrometry

Substances

Magnetite Nanoparticles
Membrane Glycoproteins
Concanavalin A