Abstract
NAC (nascent polypeptide associated complex) is a heterodimer consisting of an α and a β subunit. skNAC (skeletal and heart muscle-specific form of αNAC) is a variant of αNAC, which is induced in muscle differentiation and regeneration. We show here that skNAC expression is regulated by p38 MAPK, which plays a crucial role in myogenesis. Furthermore, inhibition of skNAC expression in myoblasts via specific siRNAs might lead to disturbed incorporation of myosin heavy chain (MyHC) into sarcomeres, however, it has no inhibitory effect on absolute MyHC protein levels. Taken together, our data suggest that skNAC regulates specific aspects of myogenesis.
Copyright © 2011 Elsevier GmbH. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Differentiation
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Cells, Cultured
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Cycloheximide / pharmacology
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Dactinomycin / pharmacology
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Mice
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Molecular Chaperones / antagonists & inhibitors
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Molecular Chaperones / genetics
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Molecular Chaperones / metabolism*
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Muscle Development
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Muscle, Skeletal / cytology
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Muscle, Skeletal / drug effects
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Muscle, Skeletal / metabolism*
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Myoblasts / cytology
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Myoblasts / drug effects
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Myoblasts / metabolism*
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Myosin Heavy Chains / metabolism
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Protein Kinase Inhibitors / pharmacology
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RNA, Small Interfering / genetics
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Regeneration
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Sarcomeres / metabolism
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Signal Transduction
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p38 Mitogen-Activated Protein Kinases / antagonists & inhibitors
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p38 Mitogen-Activated Protein Kinases / metabolism
Substances
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Molecular Chaperones
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Protein Kinase Inhibitors
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RNA, Small Interfering
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nascent-polypeptide-associated complex
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Dactinomycin
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Cycloheximide
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p38 Mitogen-Activated Protein Kinases
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Myosin Heavy Chains