Abstract
Monoclonal antibodies are frequently used as organelle-specific markers without identifying the specific antigen recognized. We have purified the protein recognized by a Golgi-specific monoclonal antibody 53FC3 (Burke, B., Griffiths, G., Reggio, H., Louvard, D., and Warren, G. (1982) EMBO J. 1, 1621-1628). Peptide microsequencing suggested that this antigen is mannosidase II, which was confirmed by cross-immunoprecipitation with an anti-mannosidase II antibody and by precipitation of mannosidase activity with the monoclonal antibody. Mannosidase II was found to exist normally as a disulfide-linked dimer.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies, Monoclonal / immunology*
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Antigens / immunology*
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Cloning, Molecular
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Disulfides
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Golgi Apparatus / immunology*
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Immunosorbent Techniques
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Macromolecular Substances
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Mannosidases / chemistry
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Mannosidases / genetics
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Mannosidases / immunology*
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Mice
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Mice, Inbred BALB C
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Molecular Sequence Data
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Molecular Weight
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Octoxynol
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Polyethylene Glycols / pharmacology
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Rats
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Trypsin / pharmacology
Substances
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Antibodies, Monoclonal
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Antigens
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Disulfides
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Macromolecular Substances
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Polyethylene Glycols
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Octoxynol
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Mannosidases
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mannosyl-oligosaccharide 1,3 - 1,6-alpha-mannosidase
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Trypsin