Abstract
To address its value as a screening tool in the development of antiviral drugs, a recombinant influenza virus expressing green fluorescent protein (rPR8-GFP virus) was investigated in vitro and in vivo. The inhibition of viral growth by a neuraminidase inhibitor in the cells or lower respiratory tracts of mice could be visualized by the level of fluorescence. In addition, the rPR8-GFP virus exhibited high pathogenicity in mice. Taken together, these results suggest that the rPR8-GFP virus can be a useful tool for the rapid identification of antiviral drugs active against influenza viruses.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antiviral Agents / pharmacology*
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Drug Evaluation, Preclinical / methods*
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Enzyme Inhibitors / pharmacology
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Female
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism*
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Humans
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Influenza A Virus, H1N1 Subtype / drug effects*
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Influenza A Virus, H1N1 Subtype / genetics
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Influenza A Virus, H1N1 Subtype / pathogenicity
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Influenza A Virus, H1N1 Subtype / physiology
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Influenza A virus / drug effects
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Influenza A virus / genetics
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Influenza A virus / pathogenicity
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Influenza A virus / physiology
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Influenza, Human / drug therapy
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Influenza, Human / virology*
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Mice
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Mice, Inbred BALB C
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Virulence
Substances
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Antiviral Agents
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Enzyme Inhibitors
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Green Fluorescent Proteins