Abstract
Protein acetylation emerged as a key regulatory mechanism for many cellular processes. We used genetic analysis of Saccharomyces cerevisiae to identify Esa1 as a histone acetyltransferase required for autophagy. We further identified the autophagy signaling component Atg3 as a substrate for Esa1. Specifically, acetylation of K19 and K48 of Atg3 regulated autophagy by controlling Atg3 and Atg8 interaction and lipidation of Atg8. Starvation induced transient K19-K48 acetylation through spatial and temporal regulation of the localization of acetylase Esa1 and the deacetylase Rpd3 on pre-autophagosomal structures (PASs) and their interaction with Atg3. Attenuation of K19-K48 acetylation was associated with attenuation of autophagy. Increased K19-K48 acetylation after deletion of the deacetylase Rpd3 caused increased autophagy. Thus, protein acetylation contributes to control of autophagy.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylation
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Autophagy*
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Autophagy-Related Protein 8 Family
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Autophagy-Related Proteins
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Carbohydrate Epimerases / genetics
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Carbohydrate Epimerases / metabolism
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Histone Acetyltransferases / genetics
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Histone Acetyltransferases / metabolism*
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Histone Deacetylases / genetics
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Histone Deacetylases / metabolism
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Microtubule-Associated Proteins / metabolism
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Mutation
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Phagosomes / metabolism
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Protein Processing, Post-Translational
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Recombinant Fusion Proteins / metabolism
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Saccharomyces cerevisiae / enzymology
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / physiology*
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Saccharomyces cerevisiae Proteins / chemistry
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism*
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Ubiquitin-Conjugating Enzymes / chemistry
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Ubiquitin-Conjugating Enzymes / genetics
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Ubiquitin-Conjugating Enzymes / metabolism*
Substances
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ATG8 protein, S cerevisiae
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Autophagy-Related Protein 8 Family
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Autophagy-Related Proteins
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Microtubule-Associated Proteins
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Recombinant Fusion Proteins
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Saccharomyces cerevisiae Proteins
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Esa1 protein, S cerevisiae
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Histone Acetyltransferases
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Ubiquitin-Conjugating Enzymes
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RPD3 protein, S cerevisiae
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Histone Deacetylases
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Carbohydrate Epimerases
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L-ribulosephosphate 4-epimerase
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ATG3 protein, S cerevisiae