Abstract
Mitotic exit integrates the reversal of the phosphorylation events initiated by mitotic kinases with a controlled cytokinesis event that cleaves the cell in two. The mitotic exit network (MEN) of budding yeast regulates both processes, whereas the fission yeast equivalent, the septum initiation network (SIN), controls only the execution of cytokinesis. The components and architecture of the SIN and MEN are highly conserved. At present, it is assumed that the functions of the core SIN-MEN components are restricted to their characterized roles at the end of mitosis. We now show that the NDR (nuclear Dbf2-related) kinase component of the fission yeast SIN, Sid2-Mob1, acts independently of the other known SIN components in G2 phase of the cell cycle to control the timing of mitotic commitment. Sid2-Mob1 promotes mitotic commitment by directly activating the NIMA (Never In Mitosis)-related kinase Fin1. Fin1's activation promotes its own destruction, thereby making Fin1 activation a transient feature of G2 phase. This spike of Fin1 activation modulates the activity of the Pom1/Cdr1/Cdr2 geometry network towards Wee1.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Cell Cycle Proteins / genetics
-
Cell Cycle Proteins / metabolism*
-
Cytokinesis*
-
Cytoskeletal Proteins / antagonists & inhibitors
-
Cytoskeletal Proteins / genetics
-
Cytoskeletal Proteins / metabolism*
-
Enzyme Activation
-
Enzyme Inhibitors / pharmacology
-
G2 Phase
-
Mitosis*
-
Mutation
-
Nuclear Proteins / genetics
-
Nuclear Proteins / metabolism*
-
Phosphorylation
-
Protein Kinases / genetics
-
Protein Kinases / metabolism*
-
Protein Serine-Threonine Kinases / metabolism
-
Protein-Tyrosine Kinases / genetics
-
Protein-Tyrosine Kinases / metabolism*
-
Saccharomyces cerevisiae Proteins / antagonists & inhibitors
-
Saccharomyces cerevisiae Proteins / genetics
-
Saccharomyces cerevisiae Proteins / metabolism*
-
Schizosaccharomyces / drug effects
-
Schizosaccharomyces / enzymology*
-
Schizosaccharomyces / genetics
-
Schizosaccharomyces / growth & development
-
Schizosaccharomyces pombe Proteins / antagonists & inhibitors
-
Schizosaccharomyces pombe Proteins / genetics
-
Schizosaccharomyces pombe Proteins / metabolism*
-
Serine
-
Signal Transduction*
-
Time Factors
Substances
-
Cell Cycle Proteins
-
Cytoskeletal Proteins
-
Enzyme Inhibitors
-
FIN1 protein, S cerevisiae
-
Nuclear Proteins
-
Saccharomyces cerevisiae Proteins
-
Schizosaccharomyces pombe Proteins
-
Serine
-
Protein Kinases
-
Pom1 protein, S pombe
-
Sid2 protein, S pombe
-
cdr1 protein, S pombe
-
wee1 protein, S pombe
-
Protein-Tyrosine Kinases
-
Cdr2 protein, S pombe
-
Protein Serine-Threonine Kinases