Abstract
TEM-1 is the dominant β-lactamase of Haemophilus influenzae and can be located on small plasmids. Three distinct plasmids with sizes from 4,304 to 5,646 nucleotides (nt) were characterized: pA1606, pA1209, and pPN223. In addition to TEM-1 and a replication enzyme of the Rep 3 superfamily, pA1606 carries a Tn3 resolvase gene and pA1606 and pA1209 carry an open reading frame (ORF) similar to a plasmid recombination enzyme gene described in Gram-positive bacteria. The plasmids transformed strain Rd to the ampicillin-resistant phenotype.
MeSH terms
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Ampicillin / pharmacology
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Base Sequence
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Haemophilus influenzae / genetics*
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Haemophilus influenzae / isolation & purification
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Open Reading Frames
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Plasmids*
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RNA-Dependent RNA Polymerase / genetics
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Recombinases / genetics
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Transformation, Bacterial
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Transposon Resolvases / genetics
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beta-Lactam Resistance / genetics*
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beta-Lactamases / genetics*
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beta-Lactams / pharmacology
Substances
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Recombinases
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beta-Lactams
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Ampicillin
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Tn3 resolvase
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Transposon Resolvases
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RNA-Dependent RNA Polymerase
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beta-Lactamases
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beta-lactamase TEM-1
Associated data
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GENBANK/JQ611726
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GENBANK/JQ611727
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GENBANK/JQ783055