Abstract
The purpose of the present study was to use zebrafish as a model to investigate how vitamin D and its receptors interact to control Ca(2+) uptake function. Low-Ca(2+) fresh water stimulated Ca(2+) influx and expressions of epithelial calcium channel (ecac), vitamin D-25-hydroxylase (cyp2r1), vitamin D receptor a (vdra), and vdrb in zebrafish. Exogenous vitamin D increased Ca(2+) influx and expressions of ecac and 25-hydroxyvitamin D(3)-24-hydroxylase (cyp24a1), but downregulated 1α-OHase (cyp27b1) with no effects on other Ca(2+) transporters. Morpholino oligonucleotide knockdown of VDRa, but not VDRb, was found as a consequence of calcium uptake inhibition by knockdown of ecac, and ossification of vertebrae is impaired. Taken together, vitamin D-VDRa signaling may stimulate Ca(2+) uptake by upregulating ECaC in zebrafish, thereby clarifying the Ca(2+)-handling function of only a VDR in teleosts. Zebrafish may be useful as a model to explore the function of vitamin D-VDR signaling in Ca(2+) homeostasis and the related physiological processes in vertebrates.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Calcium / metabolism*
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DNA Primers
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Gene Knockdown Techniques
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Immunohistochemistry
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Molecular Sequence Data
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RNA, Messenger / genetics
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Real-Time Polymerase Chain Reaction
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Receptors, Calcitriol / chemistry
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Receptors, Calcitriol / genetics
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Receptors, Calcitriol / physiology*
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Reverse Transcriptase Polymerase Chain Reaction
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Sequence Homology, Amino Acid
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Sodium-Potassium-Exchanging ATPase / metabolism
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TRPV Cation Channels / genetics
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TRPV Cation Channels / physiology
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Vitamin D / genetics
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Vitamin D / physiology*
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Zebrafish
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Zebrafish Proteins / genetics
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Zebrafish Proteins / physiology
Substances
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DNA Primers
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RNA, Messenger
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Receptors, Calcitriol
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TRPV Cation Channels
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Zebrafish Proteins
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trpv6 protein, zebrafish
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Vitamin D
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Sodium-Potassium-Exchanging ATPase
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Calcium
Grants and funding
This study was financially supported by the grant to P.P.H. from the National Science Council, Taiwan, R.O.C. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.