Variation of intragenic tandem repeat tract of tolA modulates Escherichia coli stress tolerance

PLoS One. 2012;7(10):e47766. doi: 10.1371/journal.pone.0047766. Epub 2012 Oct 19.

Abstract

In recent work we discovered that the intragenic tandem repeat (TR) region of the tolA gene is highly variable among different Escherichia coli strains. The aim of this study was therefore to investigate the biological function and dynamics of TR variation in E. coli tolA. The biological impact of TR variation was examined by comparing the ability of a set of synthetic tolA variants with in frame repeat copies varying from 2 to 39 to rescue the altered susceptibility of an E. coli ΔtolA mutant to deoxycholic acid, sodium dodecyl sulfate, hyperosmolarity, and infection with filamentous bacteriophage. Interestingly, although each of the TolA variants was able to at least partly rescue the ΔtolA mutant, the extent was clearly dependent on both the repeat number and the type of stress imposed, indicating the existence of opposing selective forces with regard to the optimal TR copy number. Subsequently, TR dynamics in a clonal population were assayed, and we could demonstrate that TR contractions are RecA dependent and enhanced in a DNA repair deficient uvrD background, and can occur at a frequency of 6.9×10(-5).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Coliphages / physiology
  • DNA Copy Number Variations
  • DNA Helicases / deficiency
  • DNA Helicases / genetics
  • Deoxycholic Acid / pharmacology
  • Escherichia coli / drug effects
  • Escherichia coli / genetics*
  • Escherichia coli / metabolism
  • Escherichia coli / virology
  • Escherichia coli Proteins / genetics*
  • Escherichia coli Proteins / metabolism
  • Genetic Variation*
  • Osmolar Concentration
  • Rec A Recombinases / genetics
  • Rec A Recombinases / metabolism
  • Sodium Dodecyl Sulfate / pharmacology
  • Stress, Physiological / genetics*
  • Tandem Repeat Sequences*

Substances

  • Escherichia coli Proteins
  • tolA protein, E coli
  • Deoxycholic Acid
  • Sodium Dodecyl Sulfate
  • Rec A Recombinases
  • UvrD protein, E coli
  • DNA Helicases

Grants and funding

This work was financially supported by a grant from the Research Foundation Flanders (G.0289.06N); http://www.fwo.be/. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.