Genome sequence and characterization of a Rhodococcus equi phage REQ1

Virus Genes. 2013 Jun;46(3):588-90. doi: 10.1007/s11262-013-0887-1. Epub 2013 Feb 5.

Abstract

Rhodococcus equi is a pathogenic member of the Actinobacteria responsible for causing serious infections in equines. A novel Siphoviridae bacteriophage (REQ1) lytic in R. equi was isolated and characterized. The genome size of REQ1 is 51,342 bp, and its sequence shares 7 % similarity to other DNA sequence in GenBank. Putative open reading frames were identified, and their functions were identified based on their predicted amino acid similarities. REQ1 phage has a modular genome, a feature common in double-stranded DNA phages.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriophages / genetics*
  • Bacteriophages / isolation & purification
  • Bacteriophages / ultrastructure
  • DNA, Viral / chemistry*
  • DNA, Viral / genetics*
  • Genome, Viral*
  • Microscopy, Electron, Transmission
  • Molecular Sequence Data
  • Open Reading Frames
  • Rhodococcus equi / virology*
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Siphoviridae / genetics*
  • Siphoviridae / isolation & purification
  • Siphoviridae / ultrastructure

Substances

  • DNA, Viral

Associated data

  • GENBANK/JN116825
  • RefSeq/NC_016655