Structural characterisation of polysaccharides from Tricholoma matsutake and their antioxidant and antitumour activities

Food Chem. 2013 Jun 15;138(4):2242-9. doi: 10.1016/j.foodchem.2012.11.140. Epub 2013 Jan 4.

Abstract

In this study, polysaccharides from Tricholoma matsutake (TM-P) were purified using a DEAE-Sepharose fast flow column and three polysaccharide fractions (TM-P1, TM-P2 and TM-P3) were obtained. The chemical composition and structural characteristics of TM-P2 were quite different from those of TM-P1 and TM-P3. TM-P2 consisted of glucose, galactose and mannose with a molar ratio of 5.9:1.1:1.0. The glycosidic linkages were mainly composed of 1,6- and 1-linked glucose. Furthermore, TM-P2 showed the strongest in vitro antioxidant and antitumour activities. The oxygen radical absorbance capacity (ORAC) of TM-P2 was 2100.44 μmol Trolox/g. The antiproliferative activities of TM-P2 (4.0mg/ml) on the growth of HepG2 and A549 cells were 67.98% and 59.04%, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / chemistry*
  • Antineoplastic Agents / pharmacology
  • Antioxidants / chemistry*
  • Antioxidants / pharmacology
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Humans
  • Molecular Structure
  • Polysaccharides / chemistry*
  • Polysaccharides / pharmacology
  • Tricholoma / chemistry*

Substances

  • Antineoplastic Agents
  • Antioxidants
  • Polysaccharides