Abstract
Many presynaptic transcripts have been observed in axons, yet their role in synapse development remains unknown. Using visually and pharmacologically isolated presynaptic terminals from dissociated rat hippocampal neurons, we found that ribosomes and β-catenin mRNA preferentially localize to recently formed boutons. Locally translated β-catenin accumulates at presynaptic terminals, where it regulates synaptic vesicle release dynamics. Thus, local translation of β-catenin is a newly described mechanism for axons to independently functionalize nerve terminals at great distances from cellular somata.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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6-Cyano-7-nitroquinoxaline-2,3-dione / pharmacology
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Animals
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Axons / drug effects
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Axons / physiology*
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Cyclodextrins / metabolism
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Cycloheximide / pharmacology
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Diffusion Chambers, Culture
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Embryo, Mammalian
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Excitatory Amino Acid Antagonists / pharmacology
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Female
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Hippocampus / cytology
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Male
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Microscopy, Confocal
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Nerve Tissue Proteins / metabolism
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Neurons / cytology*
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Neurons / drug effects
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Organ Culture Techniques
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Protein Biosynthesis / drug effects
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Protein Biosynthesis / physiology*
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Protein Synthesis Inhibitors / pharmacology
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Pyridinium Compounds / metabolism
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RNA, Messenger / metabolism
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RNA, Ribosomal / genetics
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RNA, Small Interfering / pharmacology
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Rats
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Statistics, Nonparametric
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Synaptic Vesicles / drug effects
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Synaptic Vesicles / physiology*
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Time Factors
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Tubulin / metabolism
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Valine / analogs & derivatives
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Valine / pharmacology
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beta Catenin / biosynthesis
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beta Catenin / genetics
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beta Catenin / metabolism*
Substances
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ADVASEP 7
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Bsn protein, rat
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Cyclodextrins
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Excitatory Amino Acid Antagonists
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N-(3-trimethylammoniumpropyl)-4-(6-(4-(diethylamino)phenyl)hexatrienyl)pyridinium
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Nerve Tissue Proteins
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Protein Synthesis Inhibitors
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Pyridinium Compounds
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RNA, Messenger
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RNA, Ribosomal
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RNA, Small Interfering
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Tubulin
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beta Catenin
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6-Cyano-7-nitroquinoxaline-2,3-dione
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2-amino-5-phosphopentanoic acid
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Cycloheximide
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Valine