Using A. thaliana cultured cells; we recently reported new insights regarding the effect of acute O₃ exposure. This consist in an oxidative dependent controlled cell death process involving cell shrinkage due to an early activation of anion channel (1) and a delayed activation of K(+) outward currents, but also to early events like Ca (2+) influx or singlet oxygen production possibly linked to mitochondrial dysfunction. Here we provide evidence that most of these early events act downstream of caspase-like activities as recently demonstrated for K(+) channel activation.
Keywords: Arabidopsis thaliana; caspase; ion channel; ozone; programmed cell death; reactive oxygen species; singlet oxygen.