Protein encapsulation in biodegradable polymeric nanoparticles: morphology, fluorescence behaviour and stem cell uptake

Nicoletta Rescignano; Luigi Tarpani; Roberto Tiribuzi; Simona Montesano; Sabata Martino; Loredana Latterini; Josè Maria Kenny; Ilaria Armentano

doi:10.1002/mabi.201300140

Protein encapsulation in biodegradable polymeric nanoparticles: morphology, fluorescence behaviour and stem cell uptake

Macromol Biosci. 2013 Sep;13(9):1204-12. doi: 10.1002/mabi.201300140. Epub 2013 Jun 17.

Authors

Nicoletta Rescignano¹, Luigi Tarpani, Roberto Tiribuzi, Simona Montesano, Sabata Martino, Loredana Latterini, Josè Maria Kenny, Ilaria Armentano

Affiliation

¹ Materials Engineering Center, UdR INSTM, University of Perugia, Str. Pentima 4, 05100, Terni, Italy; Institute of Polymer Science and Technology, CSIC, Juan de la Cierva 3, 28006, Madrid, Spain.

PMID: 23776101
DOI: 10.1002/mabi.201300140

Abstract

The synthesis and characterization of new biodegradable polymeric NPs loaded with bovine serum albumin marked with fluorescein isothiocyanate (FITC-BSA) is reported. The protein is encapsulated in poly(D,L-lactide-co-glycolide) (PLGA) NPs by the double emulsion method with subsequent solvent evaporation. The NPs display a spherical shape with a narrow size distribution and no aggregation is observed after drying. Steady-state and time-resolved fluorescence measurements appear to be a sensitive method to investigate the protein environment on the nanometer-scale. Finally, FITC-BSA-loaded NPs are rapidly internalized in stem cells. Interestingly, 25% cells were slightly positive after 28 days.

Keywords: PLGA; fluorescence; nanoparticles; stem cells.

MeSH terms

Animals
Biocompatible Materials / pharmacology*
Biodegradation, Environmental
Bone Marrow Cells / cytology
Bone Marrow Cells / drug effects
Bone Marrow Cells / metabolism
Calorimetry, Differential Scanning
Cattle
Cells, Cultured
Fluorescein-5-isothiocyanate / analogs & derivatives
Fluorescein-5-isothiocyanate / metabolism
Humans
Immobilized Proteins / metabolism*
Lactic Acid / chemistry*
Mesenchymal Stem Cells / cytology*
Mesenchymal Stem Cells / drug effects
Mesenchymal Stem Cells / metabolism
Nanoparticles / chemistry*
Nanoparticles / ultrastructure
Polyglycolic Acid / chemistry*
Polylactic Acid-Polyglycolic Acid Copolymer
Serum Albumin, Bovine / metabolism
Spectrometry, Fluorescence
Temperature
Thermogravimetry

Substances

Biocompatible Materials
Immobilized Proteins
fluorescein isothiocyanate bovine serum albumin
Polylactic Acid-Polyglycolic Acid Copolymer
Polyglycolic Acid
Serum Albumin, Bovine
Lactic Acid
Fluorescein-5-isothiocyanate