Solid-phase synthesis of molecularly imprinted nanoparticles for protein recognition

Serena Ambrosini; Selim Beyazit; Karsten Haupt; Bernadette Tse Sum Bui

doi:10.1039/c3cc41701h

Solid-phase synthesis of molecularly imprinted nanoparticles for protein recognition

Chem Commun (Camb). 2013 Aug 4;49(60):6746-8. doi: 10.1039/c3cc41701h.

Authors

Serena Ambrosini¹, Selim Beyazit, Karsten Haupt, Bernadette Tse Sum Bui

Affiliation

¹ Compiègne University of Technology, CNRS Enzyme and Cell Engineering Laboratory, Rue Roger Couttolenc, CS 60319, 60203 Compiègne Cedex, France.

PMID: 23785709
DOI: 10.1039/c3cc41701h

Abstract

A solid-phase synthesis approach to prepare molecularly imprinted polymer nanoparticles (MIP-NPs) specific for trypsin is described. The protein is immobilized on a solid support upon which thermoresponsive MIP-NPs are synthesized. The MIP-NPs are released by a simple temperature change, resulting in synthetic antibody mimics exhibiting high specificity and selectivity for trypsin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adsorption
Molecular Imprinting / methods*
Nanoparticles / chemistry*
Solid-Phase Synthesis Techniques / methods*
Trypsin / analysis*

Substances

Trypsin