p66Shc, mitochondria, and the generation of reactive oxygen species

Methods Enzymol. 2013:528:99-110. doi: 10.1016/B978-0-12-405881-1.00006-9.

Abstract

Reactive oxygen species (ROS), mainly originated from mitochondrial respiration, are critical inducers of oxidative damage and involved in tissue dysfunction. It is not clear, however, whether oxidative stress is the result of an active gene program or it is the by-product of physiological processes. Recent findings demonstrate that ROS are produced by mitochondria in a controlled way through specialized enzymes, including p66Shc, and take part in cellular process aimed to ensure adaptation and fitness. Therefore, genes generating specifically ROS are selected determinants of life span in response to different environmental conditions.

Keywords: Aging; Apoptosis; Mitochondria; Oxidative stress.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / drug effects
  • Body Temperature Regulation / physiology
  • Cyclosporine / pharmacology
  • Electrochemical Techniques
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Ethylmaleimide / pharmacology
  • Humans
  • Mice
  • Mitochondria, Liver / drug effects*
  • Mitochondria, Liver / metabolism
  • Mitochondrial Membrane Transport Proteins / antagonists & inhibitors
  • Mitochondrial Membrane Transport Proteins / metabolism
  • Mitochondrial Permeability Transition Pore
  • Oxidation-Reduction
  • Oxidative Stress
  • Protein Isoforms / biosynthesis
  • Protein Isoforms / genetics
  • Protein Isoforms / pharmacology
  • Reactive Oxygen Species / agonists*
  • Reactive Oxygen Species / metabolism
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / genetics
  • Recombinant Proteins / pharmacology
  • Shc Signaling Adaptor Proteins / biosynthesis
  • Shc Signaling Adaptor Proteins / genetics
  • Shc Signaling Adaptor Proteins / pharmacology*
  • Signal Transduction
  • Src Homology 2 Domain-Containing, Transforming Protein 1

Substances

  • Mitochondrial Membrane Transport Proteins
  • Mitochondrial Permeability Transition Pore
  • Protein Isoforms
  • Reactive Oxygen Species
  • Recombinant Proteins
  • SHC1 protein, human
  • Shc Signaling Adaptor Proteins
  • Src Homology 2 Domain-Containing, Transforming Protein 1
  • Cyclosporine
  • Ethylmaleimide