Overexpression of stearoyl-CoA desaturase 1 in bone-marrow mesenchymal stem cells increases osteogenesis

Panminerva Med. 2013 Sep;55(3):283-9.

Abstract

Aim: The goal of this study was to examine the relationship between stearoyl-CoA desaturase 1 (SCD1) and osteogenesis in bone marrow mesenchymal stem cells (BM-MSCs).

Methods: SCD1 was overexpressed in BM-MSCs using lentiviral transduction. Then, osteogenesis was induced by low glucose DMEM (10% fetal bovine serum). Alkaline phosphatase (ALP) activity assays, Western blot analyses of SCD1 and osteocalcin, and stainings for ALP and alizarin red were done to assess the extent of osteogenesis in BM-MSCs.

Results: ALP activity was markedly higher in the SCD1 overexpressing cells compared with cells transduced with empty vector or control (untransduced) cells at 1 and 2 weeks after osteogenic induction. By contrast, ALP activities were comparable between control cells and cells transduced with empty vector. Western blot analysis demonstrated that osteocalcin levels were higher in the SCD1 overexpressing cells, compared with cells transduced with empty vector or control cells, at 1 and 2 weeks after osteogenic induction. These findings were corroborated by stainings for ALP and alizarin red that demonstrated a more active osteogenesis in the SCD1 overexpressing cells 2 weeks after osteogenic induction.

Conclusion: Increased levels of SCD1 enhance osteogenesis in BM-MSCs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaline Phosphatase / metabolism
  • Animals
  • Bone Marrow Cells / enzymology*
  • Genetic Vectors
  • HEK293 Cells
  • Humans
  • Lentivirus / genetics
  • Mesenchymal Stem Cells / enzymology*
  • Mice
  • Osteoblasts / enzymology*
  • Osteocalcin / metabolism
  • Osteogenesis*
  • Stearoyl-CoA Desaturase / genetics
  • Stearoyl-CoA Desaturase / metabolism*
  • Time Factors
  • Transduction, Genetic
  • Transfection
  • Up-Regulation

Substances

  • Osteocalcin
  • Scd1 protein, mouse
  • Stearoyl-CoA Desaturase
  • Alkaline Phosphatase