Expression stabilities of candidate reference genes for RT-qPCR under different stress conditions in soybean

Shuhua Ma; Hongwei Niu; Chunji Liu; Jie Zhang; Chunyan Hou; Dongmei Wang

doi:10.1371/journal.pone.0075271

Expression stabilities of candidate reference genes for RT-qPCR under different stress conditions in soybean

PLoS One. 2013 Oct 4;8(10):e75271. doi: 10.1371/journal.pone.0075271. eCollection 2013.

Authors

Shuhua Ma¹, Hongwei Niu, Chunji Liu, Jie Zhang, Chunyan Hou, Dongmei Wang

Affiliation

¹ College of Life Science, Agricultural University of Hebei, Baoding, Hebei Province, China.

Abstract

Due to its accuracy, sensitivity and high throughput, real time quantitative PCR (RT-qPCR) has been widely used in analysing gene expression. The quality of data from such analyses is affected by the quality of reference genes used. Expression stabilities for nine candidate reference genes widely used in soybean were evaluated under different stresses in this study. Our results showed that EF1A and ACT11 were the best under salinity stress, TUB4, TUA5 and EF1A were the best under drought stress, ACT11 and UKN2 were the best under dark treatment, and EF1B and UKN2 were the best under virus infection. EF1B and UKN2 were the top two genes which can be reliably used in all of the stress conditions assessed.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gene Expression Regulation, Plant / genetics
Gene Expression Regulation, Plant / physiology
Glycine max / genetics*
Plant Proteins / genetics*
Reverse Transcriptase Polymerase Chain Reaction / methods*

Substances

Plant Proteins

Grants and funding

This work was supported by the National Natural Science Foundation of China (no. 30971706) and by the Natural Science Foundation of Hebei Province (no. C2008000321). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.