Two-color in situ hybridization of whole-mount mouse embryos

Kristin K Biris; Terry P Yamaguchi

doi:10.1007/978-1-60327-292-6_2

Two-color in situ hybridization of whole-mount mouse embryos

Methods Mol Biol. 2014:1092:17-30. doi: 10.1007/978-1-60327-292-6_2.

Authors

Kristin K Biris¹, Terry P Yamaguchi

Affiliation

¹ Frederick National Lab of Cancer Research, NCI, NIH, Frederick, MD, USA.

Abstract

RNA in situ hybridization is a powerful technique used to identify the spatial localization of a specific RNA in a tissue section or whole tissue. In this protocol, we describe a reliable method for two-color in situ hybridization that can be used to accurately assess the expression of multiple genes with contrasting or overlapping expression patterns in whole mouse embryos.

Publication types

Research Support, N.I.H., Intramural

MeSH terms

Animals
Embryo, Mammalian / cytology*
Gene Expression
In Situ Hybridization / methods*
Mice
Molecular Biology / methods
RNA / isolation & purification*
RNA Probes / chemistry
Staining and Labeling*

Substances

RNA Probes
RNA

Grants and funding

Z01 BC010345/Intramural NIH HHS/United States