Abstract
Ribonuclease mitochondrial RNA processing, a site-specific endoribonuclease involved in primer RNA metabolism in mammalian mitochondria, requires an RNA component for its activity. On the basis of copurification and selective inactivation with complementary oligonucleotides, a 135-nucleotide RNA species, not encoded in the mitochondrial genome, is identified as the RNA moiety of the endoribonuclease. This finding implies transport of a nucleus-encoded RNA, essential for organelle DNA replication, to the mitochondrial matrix.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Animals
-
Base Sequence
-
Cell Nucleus / physiology*
-
Chemical Phenomena
-
Chemistry
-
Drug Resistance
-
Endonucleases / isolation & purification
-
Endonucleases / metabolism
-
Enzyme Activation / drug effects
-
Genetic Code*
-
Humans
-
Mammals / genetics*
-
Mammals / metabolism
-
Micrococcal Nuclease / pharmacology
-
Mitochondria / metabolism*
-
Oligonucleotides / pharmacology
-
Organoids / physiology
-
RNA / biosynthesis*
-
RNA / genetics
-
RNA / isolation & purification
-
RNA / physiology
-
Ribonucleases / metabolism
-
Subcellular Fractions / metabolism
Substances
-
Oligonucleotides
-
RNA
-
Endonucleases
-
Ribonucleases
-
Micrococcal Nuclease