Ustilaginoidea virens is the causal agent of false smut disease of rice. The dsRNA-Ls from a U. virens strain of Uv0901 containing three dsRNA bands were separated and sequenced. The dsRNA-Ls, with molecular weight of 5 kbp, were demonstrated to be two dsRNA segments referenced as dsRNA-L1 and dsRNA-L2. The two dsRNAs each contained two overlapped open reading frames, and showed significant similarity to those of capsid protein and RNA-directed RNA polymerase, respectively, of members of the family Totiviridae. Homology research and phylogenic analysis indicated that the dsRNA-L1 was a conspecific species of the previous reported Ustilaginoidea virens RNA virus 1, named Ustilaginoidea virens RNA virus Uv0901, and the dsRNA-L2 was a new number of the Victorivirus in the family Totiviridae, designed as Ustilaginoidea virens RNA virus 3.