High-level exogenous trans10, cis12 conjugated linoleic acid plays an anti-lipogenesis role in bovine mammary epithelial cells

Anim Sci J. 2014 Jul;85(7):744-50. doi: 10.1111/asj.12204. Epub 2014 May 5.

Abstract

Primary bovine mammary epithelial cells (BMECs) were treated by 0, 37.5, 75, 112.5, 150 μmol/L trans10, cis12 conjugated linoleic acid (CLA) to evaluate the effects of different level trans10, cis12 CLA on lipogenesis in BMEC. Addition of 75-150 μmol/L trans10, cis12 CLA reduced significantly the triacylglycerol (TAG) content (P<0.05), but did not have inhibiting action on cell proliferation (P>0.05). Treatment with 150 μmol/L trans10, cis12 CLA for 48 h resulted in a 17.1% reduction (P<0.0001) of medium chain fatty acids (MCFA, C14<C<C16), a 26.5% reduction (P<0.0001) of unsaturated fatty acids (UFA) and a corresponding reduction of the mRNA abundance of acetyl coenzyme A (acetylCoA) carboxylase (ACC) (P=0.046), fatty acid synthase (FAS) (P=0.017) and stearoylCoA desaturase1 (SCD1) (P=0.002). Another finding was that trans10, cis12 CLA elevated expression of diacylglycerol acyltransferase2 (DGAT2) (P=0.020) and long chain acylCoA synthetases (ACSL) (P=0.032). In conclusion, higher trans10, cis12 CLA, not low trans10, cis12 CLA, inhibited milk fat synthesis and changed fatty acid composition by regulating the expression of FAS, ACC, SCD1, DGAT2 and ACSL.

Keywords: bovine; cis12 CLA; fatty acid composition; mammary epithelial cells; trans10; triacylglycerol.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetyl-CoA Carboxylase / genetics
  • Acetyl-CoA Carboxylase / metabolism
  • Animals
  • Cattle
  • Cell Proliferation / drug effects
  • Cells, Cultured
  • Coenzyme A Ligases / metabolism
  • Diacylglycerol O-Acyltransferase / metabolism
  • Dose-Response Relationship, Drug
  • Epithelial Cells / cytology
  • Epithelial Cells / metabolism*
  • Fatty Acid Synthases / metabolism
  • Fatty Acids / metabolism
  • Fatty Acids, Unsaturated / metabolism
  • Female
  • Linoleic Acids, Conjugated / pharmacology*
  • Lipogenesis / drug effects*
  • Mammary Glands, Animal / cytology*
  • Milk / metabolism*
  • RNA, Messenger / metabolism
  • Stearoyl-CoA Desaturase / metabolism
  • Triglycerides / metabolism

Substances

  • Fatty Acids
  • Fatty Acids, Unsaturated
  • Linoleic Acids, Conjugated
  • RNA, Messenger
  • Triglycerides
  • trans-10,cis-12-conjugated linoleic acid
  • Stearoyl-CoA Desaturase
  • Diacylglycerol O-Acyltransferase
  • Fatty Acid Synthases
  • Coenzyme A Ligases
  • Acetyl-CoA Carboxylase