Lipomyces chichibuensis sp. nov., isolated in Japan, and reidentification of the type strains of Lipomyces kononenkoae and Lipomyces spencermartinsiae

Int J Syst Evol Microbiol. 2014 Aug;64(Pt 8):2566-2572. doi: 10.1099/ijs.0.059972-0. Epub 2014 May 8.

Abstract

We isolated two strains of a novel Lipomyces species from soil collected in Chichibu forest, Saitama prefecture, Japan. Based on their morphological and biochemical characteristics, along with multilocus sequence typing using the D1/D2 domain of the large-subunit (LSU) rRNA gene, the internal transcribed spacer (ITS) region and the translation elongation factor 1 alpha gene (EF-1α), the two strains were shown to represent a novel species of the genus Lipomyces, described as Lipomyces chichibuensis sp. nov. (type strain CB08-2(T) = NBRC 109582(T) = CBS 12929(T); Mycobank no. MB808164). In addition, we reidentified the type strains of Lipomyces kononenkoae and Lipomyces spencermartinsiae maintained in culture collections based on phenotypic characters and/or DNA-DNA hybridization to ensure correct future identification of species of the genus Lipomyces. The correct type strains of L. kononenkoae and L. spencermartinsiae are NBRC 107661(T) ( = CBS 2514(T)) and NBRC 10376(T) ( = CBS 5608(T)), respectively.

MeSH terms

  • DNA, Fungal / genetics
  • DNA, Ribosomal Spacer / genetics
  • Japan
  • Lipomyces / classification*
  • Lipomyces / genetics
  • Lipomyces / isolation & purification
  • Molecular Sequence Data
  • Mycological Typing Techniques
  • Nucleic Acid Hybridization
  • Peptide Elongation Factor 1 / genetics
  • Phylogeny*
  • Sequence Analysis, DNA
  • Soil Microbiology*

Substances

  • DNA, Fungal
  • DNA, Ribosomal Spacer
  • Peptide Elongation Factor 1

Associated data

  • GENBANK/AB828721
  • GENBANK/AB828723
  • GENBANK/AB828725