The purpose of this study was to examine the effects of 2 braking forces (8.7 and 11% of body mass, BM) on Wingate test performance, peak lactate ([La]pk), peak heart rate (HRpk), and rate of perceived exertion (RPE). Sixteen male physical education students (age: 22.7 ± 1.3 years, height: 1.81 ± 0.07 m, BM: 74.3 ± 9.6 kg) performed, in a randomized order, 2 Wingate tests at 8.7% BM and 2 Wingate tests at 11% BM on a Monark cycle ergometer on 4 separate sessions. The results showed that the reliability level of mechanical measures was not affected by the braking force and was relatively similar for each variable in both braking forces (0.886 < ICC < 0.985). In addition, peak power, mean power, fatigue slope, and RPE were significantly higher (8.2, 7.0, 11.9, and 4.1%, respectively, all < 0.05) using a braking force of 11% BM compared with 8.7% BM, whereas there was no significant effect of braking force on [La]pk and HRpk. In conclusion, the results of this study suggested that the reliability of the Wingate test does not depend on the used load, and a braking force of 11% BM is more optimal for power output during Wingate test in active adults.