Molecular characterisation of an endornavirus infecting the phytopathogen Sclerotinia sclerotiorum

Virus Res. 2014 Aug 30:189:303-9. doi: 10.1016/j.virusres.2014.06.010. Epub 2014 Jun 28.

Abstract

The complete sequence and genome organisation of an endornavirus from the phytopathogenic fungus Sclerotinia sclerotiorum isolate 11691 was described and the name Sclerotinia sclerotiorum endornavirus 1 (SsEV1/11691) proposed. The genome is 10,513 nucleotides (nts) long with a single open reading frame (ORF) that codes for a single polyprotein of 3459 amino acid (aa) residues. The polyprotein contains cysteine-rich region (CRR), viral methyltransferase (MTR), putative DEXDc, viral helicase (Hel), phytoreo_S7 (S7) and RNA-dependent RNA polymerase (RdRp) domains. The polyprotein and the conserved domains are phylogenetically related to endornaviruses. However, the coding strand of SsEV1/11691 does not contain a site-specific nick characteristic of most previously described endornaviruses. The elimination of SsEV1/11691 did not result in any significant changes in the host phenotype and virulence.

Keywords: Endornaviridae; Endornavirus; Mitovirus; Sclerotinia sclerotiorum; SsEV1; dsRNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Ascomycota / virology*
  • Cluster Analysis
  • Genome, Viral*
  • Molecular Sequence Data
  • Open Reading Frames
  • Phylogeny
  • Polyproteins / genetics
  • RNA Viruses / genetics*
  • RNA Viruses / isolation & purification
  • RNA, Viral / genetics*
  • Sequence Analysis, DNA*
  • Sequence Homology, Amino Acid
  • Viral Proteins / genetics

Substances

  • Polyproteins
  • RNA, Viral
  • Viral Proteins

Associated data

  • GENBANK/KJ123645
  • GENBANK/KJ462509
  • RefSeq/NC_023893