Biomimetic alginate/polyacrylamide porous scaffold supports human mesenchymal stem cell proliferation and chondrogenesis

Mater Sci Eng C Mater Biol Appl. 2014 Sep:42:622-8. doi: 10.1016/j.msec.2014.06.013. Epub 2014 Jun 19.

Abstract

We describe the development of alginate/polyacrylamide (ALG/PAAm) porous hydrogels based on interpenetrating polymer network structure for human mesenchymal stem cell proliferation and chondrogenesis. Three ALG/PAAm hydrogels at molar ratios of 10/90, 20/80, and 30/70 were prepared and characterized with enhanced elastic and rubbery mechanical properties, which are similar to native human cartilage tissues. Their elasticity and swelling properties were also studied under different physiological pH conditions. Finally, in vitro tests demonstrated that human mesenchymal stem cells could proliferate on the as-synthesized hydrogels with improved alkaline phosphatase activities. These results suggest that ALG/PAAm hydrogels may be a promising biomaterial for cartilage tissue engineering.

Keywords: Alginate; Chondrogenesis; Hydrogel; Mesenchymal stem cell; Polyacrylamide; Proliferation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acrylic Resins / chemistry*
  • Acrylic Resins / pharmacology
  • Alginates / chemistry*
  • Alginates / pharmacology
  • Biomimetic Materials / chemistry
  • Biomimetic Materials / pharmacology
  • Cell Proliferation / drug effects*
  • Cells, Cultured
  • Chondrogenesis / drug effects*
  • Glucuronic Acid / chemistry
  • Glucuronic Acid / pharmacology
  • Hexuronic Acids / chemistry
  • Hexuronic Acids / pharmacology
  • Humans
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / drug effects*
  • Porosity
  • Tissue Scaffolds / chemistry*

Substances

  • Acrylic Resins
  • Alginates
  • Hexuronic Acids
  • Glucuronic Acid
  • polyacrylamide