Molecular cloning and functional characterization of porcine DEAD (Asp-Glu-Ala-Asp) box polypeptide 41 (DDX41)

Xinyu Zhu; Dang Wang; Huan Zhang; Yanrong Zhou; Rui Luo; Huanchun Chen; Shaobo Xiao; Liurong Fang

doi:10.1016/j.dci.2014.07.020

Molecular cloning and functional characterization of porcine DEAD (Asp-Glu-Ala-Asp) box polypeptide 41 (DDX41)

Dev Comp Immunol. 2014 Dec;47(2):191-6. doi: 10.1016/j.dci.2014.07.020. Epub 2014 Jul 30.

Authors

Xinyu Zhu¹, Dang Wang¹, Huan Zhang¹, Yanrong Zhou¹, Rui Luo¹, Huanchun Chen¹, Shaobo Xiao¹, Liurong Fang²

Affiliations

¹ State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
² State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: fanglr@mail.hzau.edu.cn.

PMID: 25086295
DOI: 10.1016/j.dci.2014.07.020

Abstract

DEAD (Asp-Glu-Ala-Asp) box polypeptide 41 (DDX41), a member of the DEXDc helicase family, was recently identified as an intracellular DNA sensor in mouse myeloid dendritic cells. In this study, porcine DDX41 (poDDX41) was cloned and its role in the type I interferon (IFN) signaling pathway was investigated in porcine kidney (PK-15) cells. Full-length poDDX41 cDNA encodes 622 amino acid residues and contains a DEADc domain and a HELICc domain. poDDX41 mRNA is widely expressed in different tissues, especially the stomach and liver. Overexpression of poDDX41 in PK-15 cells induced IFN-β by activating transcription factors IRF3 and NF-κB. Knockdown of poDDX41 with siRNA significantly reduced IFN-β expression induced by poly(dA:dT), a double-stranded DNA (dsDNA) analogue, or pseudorabies virus, a dsDNA swine virus. Therefore, poDDX41 is involved in the dsDNA- and dsDNA-virus-mediated type I IFN signaling pathway in porcine kidney cells.

Keywords: DDX41; Interferon-β; Porcine.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Cloning, Molecular*
DEAD-box RNA Helicases / antagonists & inhibitors
DEAD-box RNA Helicases / genetics*
DEAD-box RNA Helicases / immunology
DNA / genetics
DNA / immunology
DNA, Complementary / genetics
DNA, Complementary / immunology
Epithelial Cells / cytology
Epithelial Cells / immunology*
Gene Expression Regulation
Herpesvirus 1, Suid / genetics
Herpesvirus 1, Suid / immunology
Interferon Regulatory Factor-3 / genetics
Interferon Regulatory Factor-3 / immunology
Interferon-beta / genetics
Interferon-beta / immunology*
Kidney / cytology
Kidney / immunology*
Liver / immunology
Molecular Sequence Data
NF-kappa B / genetics
NF-kappa B / immunology
Open Reading Frames
Poly dA-dT / genetics
Poly dA-dT / immunology
Protein Structure, Tertiary
RNA, Small Interfering / genetics
RNA, Small Interfering / metabolism
Sequence Alignment
Sequence Homology, Amino Acid
Signal Transduction
Stomach / immunology
Swine

Substances

DNA, Complementary
Interferon Regulatory Factor-3
NF-kappa B
RNA, Small Interfering
Poly dA-dT
Interferon-beta
DNA
DEAD-box RNA Helicases