Preferential localization of type I phosphatidylinositol 4-phosphate 5-kinase γ at the periactive zone of mouse photoreceptor ribbon synapses

Hiroyuki Sakagami; Osamu Katsumata; Yoshinobu Hara; Hideaki Tamaki; Masahiro Fukaya

doi:10.1016/j.brainres.2014.08.051

Preferential localization of type I phosphatidylinositol 4-phosphate 5-kinase γ at the periactive zone of mouse photoreceptor ribbon synapses

Brain Res. 2014 Oct 24:1586:23-33. doi: 10.1016/j.brainres.2014.08.051. Epub 2014 Aug 22.

Authors

Hiroyuki Sakagami¹, Osamu Katsumata², Yoshinobu Hara², Hideaki Tamaki², Masahiro Fukaya²

Affiliations

¹ Department of Anatomy, Kitasato University School of Medicine, Sagamihara, Kanagawa 252-0374, Japan. Electronic address: sakagami@med.kitasato-u.ac.jp.
² Department of Anatomy, Kitasato University School of Medicine, Sagamihara, Kanagawa 252-0374, Japan.

PMID: 25152467
DOI: 10.1016/j.brainres.2014.08.051

Abstract

Type I phosphatidylinositol 4-phosphate 5 kinase γ (PIP5KIγ) constitutes a major pathway for the generation of phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) that regulates a variety of neuronal functions at both presynaptic and postsynaptic compartments. In this study, we examined the expression and localization of PIP5KIγ in the adult mouse retina. RT-PCR analysis revealed that PIP5KIγ_v2 was predominantly expressed in the retina while PIP5KIγ_v3 was also expressed faintly. Immunostaining of the adult mouse retina revealed intense PIP5KIγ-immunoreactivity in the inner and outer plexiform layers in a punctate manner. In the photoreceptor ribbon synapse, PIP5KIγ was highly concentrated at the periactive zone. These findings suggest that PIP5KIγ, especially PIP5KIγ_i2, is localized at the periactive zone, a functionally suitable compartment for the endocytosis of synaptic vesicles in photoreceptor ribbon synapses.

Keywords: Active zone; Endocytosis; Phosphoinositide; Photoreceptor; Retina; Ribbon synapse.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alcohol Oxidoreductases
Animals
Calbindins / metabolism
Clathrin / metabolism
Co-Repressor Proteins
DNA-Binding Proteins / metabolism
Disks Large Homolog 4 Protein
Guanylate Kinases / metabolism
Male
Membrane Proteins / metabolism
Mice
Mice, Inbred C57BL
Microscopy, Immunoelectron
Nerve Tissue Proteins / metabolism
Phosphoproteins / metabolism
Phosphotransferases (Alcohol Group Acceptor) / metabolism*
Photoreceptor Cells / metabolism*
Photoreceptor Cells / ultrastructure
Protein Kinase C / metabolism
Retina / cytology
Synapses / metabolism*
Synapses / ultrastructure
Talin / metabolism
Vesicular Inhibitory Amino Acid Transport Proteins / metabolism

Substances

Bsn protein, mouse
Calbindins
Clathrin
Co-Repressor Proteins
DNA-Binding Proteins
Disks Large Homolog 4 Protein
Dlg4 protein, mouse
Membrane Proteins
Nerve Tissue Proteins
Phosphoproteins
Talin
Vesicular Inhibitory Amino Acid Transport Proteins
Viaat protein, mouse
Alcohol Oxidoreductases
Ctbp2 protein, mouse
Phosphotransferases (Alcohol Group Acceptor)
1-phosphatidylinositol-4-phosphate 5-kinase
Protein Kinase C
Guanylate Kinases