Draft Genome Sequence of Escherichia coli Strain VKPM B-10182, Producing the Enzyme for Synthesis of Cephalosporin Acids

Andrey V Mardanov; Mikhail A Eldarov; Anna V Sklyarenko; Maria V Dumina; Alexey V Beletsky; Sergey V Yarotsky; Nikolai V Ravin

doi:10.1128/genomeA.01222-14

Draft Genome Sequence of Escherichia coli Strain VKPM B-10182, Producing the Enzyme for Synthesis of Cephalosporin Acids

Genome Announc. 2014 Nov 20;2(6):e01222-14. doi: 10.1128/genomeA.01222-14.

Authors

Andrey V Mardanov¹, Mikhail A Eldarov¹, Anna V Sklyarenko², Maria V Dumina¹, Alexey V Beletsky¹, Sergey V Yarotsky², Nikolai V Ravin³

Affiliations

¹ Centre "Bioengineering," Russian Academy of Sciences, Moscow, Russian Federation.
² State Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, Russian Federation.
³ Centre "Bioengineering," Russian Academy of Sciences, Moscow, Russian Federation nravin@biengi.ac.ru.

Abstract

Escherichia coli strain VKPM B-10182, obtained by chemical mutagenesis from E. coli strain ATCC 9637, produces cephalosporin acid synthetase employed in the synthesis of β-lactam antibiotics, such as cefazolin. The draft genome sequence of strain VKPM B-10182 revealed 32 indels and 1,780 point mutations that might account for the improvement in antibiotic synthesis that we observed.