Astrocytic TLR4 expression and LPS-induced nuclear translocation of STAT3 in the sensory circumventricular organs of adult mouse brain

Yousuke Nakano; Eriko Furube; Shoko Morita; Akio Wanaka; Toshihiro Nakashima; Seiji Miyata

doi:10.1016/j.jneuroim.2014.12.013

Astrocytic TLR4 expression and LPS-induced nuclear translocation of STAT3 in the sensory circumventricular organs of adult mouse brain

J Neuroimmunol. 2015 Jan 15:278:144-58. doi: 10.1016/j.jneuroim.2014.12.013. Epub 2014 Dec 16.

Authors

Yousuke Nakano¹, Eriko Furube¹, Shoko Morita², Akio Wanaka², Toshihiro Nakashima¹, Seiji Miyata³

Affiliations

¹ Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
² The Department of Anatomy and Neuroscience, Faculty of Medicine, Nara Medical University, Shijo-cho, Kashihara, Nara 634-8521, Japan.
³ Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan. Electronic address: smiyata@kit.ac.jp.

PMID: 25595264
DOI: 10.1016/j.jneuroim.2014.12.013

Abstract

The sensory circumventricular organs (CVOs) comprise the organum vasculosum of the lamina terminalis (OVLT), subfornical organ (SFO), and area postrema (AP) and lack the blood-brain barrier. The expression of Toll-like receptor 4 (TLR4) was seen at astrocytes throughout the sensory CVOs and at microglia in the AP and solitary nucleus around the central canal. The peripheral and central administration of lipopolysaccharide induced a similar pattern of nuclear translocation of STAT3. A microglia inhibitor minocycline largely suppressed lipopolysaccharide-induced astrocytic nuclear translocation of STAT3 in the OVLT and AP, but its effect was less in the SFO.

Keywords: Astrocyte; Brain; Fever; Inflammation; Microglia.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Astrocytes / drug effects
Astrocytes / metabolism
Brain / anatomy & histology*
Brain / drug effects
Calcium-Binding Proteins / metabolism
Circumventricular Organs / drug effects*
Dose-Response Relationship, Drug
Drug Administration Routes
Gene Expression Regulation / drug effects*
Glial Fibrillary Acidic Protein / metabolism
Laminin / metabolism
Leukocyte Common Antigens / metabolism
Lipopolysaccharides / pharmacology*
Male
Mice
Mice, Inbred ICR
Microfilament Proteins / metabolism
Microscopy, Confocal
Minocycline / pharmacology
STAT3 Transcription Factor / metabolism*
Time Factors
Toll-Like Receptor 4 / metabolism*

Substances

Aif1 protein, mouse
Calcium-Binding Proteins
Glial Fibrillary Acidic Protein
Laminin
Lipopolysaccharides
Microfilament Proteins
STAT3 Transcription Factor
Tlr4 protein, rat
Toll-Like Receptor 4
Leukocyte Common Antigens
Minocycline