Abstract
Toxoplasma gondii induces polarization of mouse macrophages, including both classically activated macrophages (M1) and alternatively activated macrophages (M2) in a genotype-related manner. Here we present a novel result that the Wh6 strain with type Chinese 1, which is predominantly prevalent in China, induces Arg1 expression in a STAT6-dependent manner in primary rat peritoneal macrophages as compared to the PRU stain with type II, which elicited a high expression of Arg1 in a C/EBPβ-dependent manner. In addition, dexamethasone inhibited Arg1 expression in rat macrophages in both treatments. Our data suggest that Arg1 expression, which is abundant in polarized M2 cells, is associated with strain/genotype differences from different pathways.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Arginase / antagonists & inhibitors
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Arginase / genetics
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Arginase / metabolism*
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Blotting, Western
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CCAAT-Enhancer-Binding Protein-beta / metabolism
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DNA, Complementary / biosynthesis
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Dexamethasone / pharmacology
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Down-Regulation
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Female
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Gene Expression Regulation, Enzymologic / genetics*
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Genotype
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Glucocorticoids / pharmacology
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Macrophages, Peritoneal / enzymology*
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Macrophages, Peritoneal / parasitology*
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Mice
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Nitric Oxide / metabolism
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RNA, Messenger / metabolism
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RNA, Protozoan / genetics
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RNA, Protozoan / isolation & purification
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Rats
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Rats, Sprague-Dawley
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Real-Time Polymerase Chain Reaction
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STAT6 Transcription Factor / genetics
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STAT6 Transcription Factor / metabolism
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Signal Transduction
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Toxoplasma / classification
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Toxoplasma / physiology*
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Up-Regulation
Substances
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CCAAT-Enhancer-Binding Protein-beta
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DNA, Complementary
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Glucocorticoids
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RNA, Messenger
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RNA, Protozoan
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STAT6 Transcription Factor
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Nitric Oxide
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Dexamethasone
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Arginase
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arginase I, rat