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Biosci Biotechnol Biochem. 2015;79(9):1438-46. doi: 10.1080/09168451.2015.1043118. Epub 2015 May 12.

Profilin 2 promotes migration, invasion, and stemness of HT29 human colorectal cancer stem cells.

Author information

1
a College of Life Sciences and Biotechnology , Korea University , Seoul 136-701 , Korea.

Abstract

We investigated the role of profilin 2 in the stemness, migration, and invasion of HT29 cancer stem cells (CSCs). Increased and decreased levels of profilin 2 significantly enhanced and suppressed the self-renewal, migration, and invasion ability of HT29 CSCs, respectively. Moreover, profilin 2 directly regulated the expression of stemness markers (CD133, SOX2, and β-catenin) and epithelial mesenchymal transition (EMT) markers (E-cadherin and snail). CD133 and β-catenin were up-regulated by overexpression of profilin 2 and down-regulated by depletion of profilin 2. SOX2 was decreased by profilin 2 depletion. E-cadherin was not influenced by profilin 2- overexpression but increased by profilin 2- knockdown. The expression of snail was suppressed by profilin 2- knockdown. We speculated that stemness and the EMT are closely linked through profilin 2-related pathways. Therefore, this study indicates that profilin 2 affects the metastatic potential and stemness of colorectal CSCs by regulating EMT- and stemness-related proteins.

KEYWORDS:

Cancer stem cells (CSCs); Invasion; Migration; Profilin 2; Stemness

PMID:
25964982
DOI:
10.1080/09168451.2015.1043118
[Indexed for MEDLINE]

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