The Complete Genome of a New Betabaculovirus from Clostera anastomosis

Feifei Yin; Zheng Zhu; Xiaoping Liu; Dianhai Hou; Jun Wang; Lei Zhang; Manli Wang; Zheng Kou; Hualin Wang; Fei Deng; Zhihong Hu

doi:10.1371/journal.pone.0132792

The Complete Genome of a New Betabaculovirus from Clostera anastomosis

PLoS One. 2015 Jul 13;10(7):e0132792. doi: 10.1371/journal.pone.0132792. eCollection 2015.

Authors

Feifei Yin¹, Zheng Zhu², Xiaoping Liu², Dianhai Hou², Jun Wang², Lei Zhang², Manli Wang², Zheng Kou², Hualin Wang², Fei Deng², Zhihong Hu²

Affiliations

¹ State Key Laboratory of Virology and China Center for Virus Culture Collection, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, PR China; School of Tropical and Laboratory Medicine, Hainan Medical University, Haikou, 571101, PR China.
² State Key Laboratory of Virology and China Center for Virus Culture Collection, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, PR China.

Abstract

Clostera anastomosis (Lepidoptera: Notodontidae) is a defoliating forest insect pest. Clostera anastomosis granulovirus-B (ClasGV-B) belonging to the genus Betabaculovirus of family Baculoviridae has been used for biological control of the pest. Here we reported the full genome sequence of ClasGV-B and compared it to other previously sequenced baculoviruses. The circular double-stranded DNA genome is 107,439 bp in length, with a G+C content of 37.8% and contains 123 open reading frames (ORFs) representing 93% of the genome. ClasGV-B contains 37 baculovirus core genes, 25 lepidopteran baculovirus specific genes, 19 betabaculovirus specific genes, 39 other genes with homologues to baculoviruses and 3 ORFs unique to ClasGV-B. Hrs appear to be absent from the ClasGV-B genome, however, two non-hr repeats were found. Phylogenetic tree based on 37 core genes from 73 baculovirus genomes placed ClasGV-B in the clade b of betabaculoviruses and was most closely related to Erinnyis ello GV (ErelGV). The gene arrangement of ClasGV-B also shared the strongest collinearity with ErelGV but differed from Clostera anachoreta GV (ClanGV), Clostera anastomosis GV-A (ClasGV-A, previously also called CaLGV) and Epinotia aporema GV (EpapGV) with a 20 kb inversion. ClasGV-B genome contains three copies of polyhedron envelope protein gene (pep) and phylogenetic tree divides the PEPs of betabaculoviruses into three major clades: PEP-1, PEP-2 and PEP/P10. ClasGV-B also contains three homologues of P10 which all harbor an N-terminal coiled-coil domain and a C-terminal basic sequence. ClasGV-B encodes three fibroblast growth factor (FGF) homologues which are conserved in all sequenced betabaculoviruses. Phylogenetic analysis placed these three FGFs into different groups and suggested that the FGFs were evolved at the early stage of the betabaculovirus expansion. ClasGV-B is different from previously reported ClasGV-A and ClanGV isolated from Notodontidae in sequence and gene arrangement, indicating the virus is a new notodontid betabaculovirus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Baculoviridae / genetics*
Baculoviridae / pathogenicity
Base Sequence
Genome, Viral*
Lepidoptera / virology*
Molecular Sequence Data
Open Reading Frames
Phylogeny
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid

Grants and funding

The research was supported in part by the Strategic Priority Research Program of the Chinese Academy of Sciences (Grant No. XDB11030400) and the National Science Foundation of China (31321001, 31200124, 31460017). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.