Complete genome sequence analysis and identification of putative metallo-beta-lactamase and SpoIIIE homologs in Bacillus cereus group phage BCP8-2, a new member of the proposed Bastille-like group

Paul Tetteh Asare; Nadeeka Bandara; Tae-Yong Jeong; Sangryeol Ryu; Jochen Klumpp; Kwang-Pyo Kim

doi:10.1007/s00705-015-2548-2

Complete genome sequence analysis and identification of putative metallo-beta-lactamase and SpoIIIE homologs in Bacillus cereus group phage BCP8-2, a new member of the proposed Bastille-like group

Arch Virol. 2015 Oct;160(10):2647-50. doi: 10.1007/s00705-015-2548-2. Epub 2015 Aug 4.

Authors

Paul Tetteh Asare¹, Nadeeka Bandara¹, Tae-Yong Jeong¹, Sangryeol Ryu^{2

3}, Jochen Klumpp⁴, Kwang-Pyo Kim⁵

Affiliations

¹ Department of Food Science and Technology, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju, Jeollabuk-do, 561-756, Korea.
² Department of Food and Animal Biotechnology, Center for Agricultural Biomaterials, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Korea.
³ Department of Agricultural Biotechnology, Center for Agricultural Biomaterials, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Korea.
⁴ Institute of Food, Nutrition and Health, ETH Zurich, Schmelzbergstrasse 7, 8092, Zurich, Switzerland.
⁵ Department of Food Science and Technology, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju, Jeollabuk-do, 561-756, Korea. kpkim@jbnu.ac.kr.

PMID: 26234184
DOI: 10.1007/s00705-015-2548-2

Abstract

Bacillus cereus group-specific bacteriophage BCP8-2 exhibits a broad lysis spectrum among food and human isolates (330/364) of B. cereus while not infecting B. subtilis (50) or B. licheniformis (12) strains. Its genome is 159,071 bp long with 220 open reading frames, including genes for putative methyltransferases, metallo-beta-lactamase, and a sporulation-related SpoIIIE homolog, as wells as 18 tRNAs. Comparative genome analysis showed that BCP8-2 is related to the recently proposed Bastille-like phages, but not with either SPO1-like or Twort-like phages of the subfamily Spounavirinae.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacillus Phages / classification
Bacillus Phages / enzymology
Bacillus Phages / genetics*
Bacillus Phages / isolation & purification*
Bacillus cereus / virology*
Base Sequence
Genome, Viral*
Molecular Sequence Data
Myoviridae / classification
Myoviridae / enzymology
Myoviridae / genetics*
Myoviridae / isolation & purification*
Open Reading Frames
Phylogeny
Viral Proteins / genetics*
Viral Proteins / metabolism
beta-Lactamases / genetics*
beta-Lactamases / metabolism

Substances

Viral Proteins
beta-Lactamases