Soluble Envelope Glycoprotein Trimers from a CD4-Independent HIV-1 Elicit Antibody-Dependent Cellular Cytotoxicity-Mediating Antibodies in Guinea Pigs

J Virol. 2015 Oct;89(20):10707-11. doi: 10.1128/JVI.01642-15. Epub 2015 Aug 5.

Abstract

CD4-independent HIV-1 variants can infect coreceptor-expressing cells lacking CD4. The envelope (Env) glycoproteins on these HIV-1 variants expose a coreceptor binding site that overlaps some CD4-induced (CD4i) epitopes. Reports have demonstrated that CD4i antibodies mediate antibody-dependent cellular cytotoxicity (ADCC). Here we investigated the immunogenicity of soluble Env trimers (sgp140) from a CD4-independent HIV-1 in guinea pigs and found that the sgp140 elicited ADCC-mediating antibodies. Therefore, these sgp140 might be useful in vaccine regimens aimed at eliciting ADCC responses.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibody-Dependent Cell Cytotoxicity / genetics*
  • Binding Sites
  • CD4 Antigens / genetics
  • CD4 Antigens / immunology
  • Epitopes / chemistry
  • Epitopes / genetics
  • Epitopes / immunology
  • Gene Expression
  • Guinea Pigs
  • HIV Antibodies / biosynthesis*
  • HIV Infections / immunology
  • HIV Infections / prevention & control*
  • HIV Infections / virology
  • HIV-1 / genetics
  • HIV-1 / immunology*
  • Humans
  • Immunization
  • Protein Binding
  • Protein Multimerization
  • Solubility
  • env Gene Products, Human Immunodeficiency Virus / administration & dosage
  • env Gene Products, Human Immunodeficiency Virus / chemistry
  • env Gene Products, Human Immunodeficiency Virus / genetics
  • env Gene Products, Human Immunodeficiency Virus / immunology*

Substances

  • CD4 Antigens
  • Epitopes
  • HIV Antibodies
  • env Gene Products, Human Immunodeficiency Virus