Generation of gene-target dogs using CRISPR/Cas9 system

Qingjian Zou; Xiaomin Wang; Yunzhong Liu; Zhen Ouyang; Haibin Long; Shu Wei; Jige Xin; Bentian Zhao; Sisi Lai; Jun Shen; Qingchun Ni; Huaqiang Yang; Huilin Zhong; Li Li; Minhua Hu; Quanjun Zhang; Zhidong Zhou; Jiaxin He; Quanmei Yan; Nana Fan; Yu Zhao; Zhaoming Liu; Lin Guo; Jiao Huang; Guanguan Zhang; Jun Ying; Liangxue Lai; Xiang Gao

doi:10.1093/jmcb/mjv061

Generation of gene-target dogs using CRISPR/Cas9 system

J Mol Cell Biol. 2015 Dec;7(6):580-3. doi: 10.1093/jmcb/mjv061. Epub 2015 Oct 12.

Authors

Qingjian Zou¹, Xiaomin Wang², Yunzhong Liu³, Zhen Ouyang¹, Haibin Long³, Shu Wei¹, Jige Xin⁴, Bentian Zhao¹, Sisi Lai¹, Jun Shen⁵, Qingchun Ni³, Huaqiang Yang¹, Huilin Zhong¹, Li Li¹, Minhua Hu³, Quanjun Zhang¹, Zhidong Zhou³, Jiaxin He³, Quanmei Yan¹, Nana Fan¹, Yu Zhao¹, Zhaoming Liu¹, Lin Guo¹, Jiao Huang¹, Guanguan Zhang³, Jun Ying⁶, Liangxue Lai⁷, Xiang Gao⁸

Affiliations

¹ CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
² Key Laboratory of Model Animal for Disease Study of Ministry of Education, Model Animal Research Center, Nanjing University, Nanjing 210061, China.
³ Guangzhou General Pharmaceutical Research Institute Co., Ltd, Guangzhou 510240, China.
⁴ Jilin Provincial Key Laboratory of Animal Embryo Engineering, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China.
⁵ Department of Radiology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, China.
⁶ Guangzhou General Pharmaceutical Research Institute Co., Ltd, Guangzhou 510240, China teddying@163.com lai_liangxue@gibh.ac.cn gaoxiang@nju.edu.cn.
⁷ CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China Jilin Provincial Key Laboratory of Animal Embryo Engineering, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China teddying@163.com lai_liangxue@gibh.ac.cn gaoxiang@nju.edu.cn.
⁸ Key Laboratory of Model Animal for Disease Study of Ministry of Education, Model Animal Research Center, Nanjing University, Nanjing 210061, China teddying@163.com lai_liangxue@gibh.ac.cn gaoxiang@nju.edu.cn.

PMID: 26459633
DOI: 10.1093/jmcb/mjv061

No abstract available

Publication types

Letter
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
CRISPR-Cas Systems*
Dogs / genetics*
Exons
Female
Gene Knockout Techniques*
Hypertrophy / genetics
Male
Molecular Sequence Data
Muscular Diseases / genetics
Myostatin / genetics*
RNA, Guide, CRISPR-Cas Systems / genetics

Substances

Myostatin
RNA, Guide, CRISPR-Cas Systems

Supplementary concepts

Myostatin-related muscle hypertrophy