Interaction between ORF24 and ORF34 in the Kaposi's Sarcoma-Associated Herpesvirus Late Gene Transcription Factor Complex Is Essential for Viral Late Gene Expression

J Virol. 2015 Oct 14;90(1):599-604. doi: 10.1128/JVI.02157-15. Print 2016 Jan 1.

Abstract

Transcription of herpesviral late genes is stimulated after the onset of viral DNA replication but otherwise restricted. Late gene expression in gammaherpesviruses requires the coordination of six early viral proteins, termed viral transactivation factors (vTFs). Here, we mapped the organization of this protein complex for Kaposi's sarcoma-associated herpesvirus. Disruption of this complex via point mutation of the interaction interface between the open reading frame 24 (ORF24) and ORF34 vTFs ablated both late gene expression and viral replication.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • DNA Mutational Analysis
  • Gene Expression Regulation, Viral*
  • Herpesvirus 8, Human / genetics*
  • Herpesvirus 8, Human / physiology
  • Humans
  • Models, Biological
  • Mutant Proteins / genetics
  • Mutant Proteins / metabolism
  • Point Mutation
  • Protein Binding
  • Protein Interaction Mapping
  • Protein Multimerization
  • Transcription, Genetic*
  • Viral Proteins / genetics
  • Viral Proteins / metabolism*
  • Virus Replication

Substances

  • Mutant Proteins
  • Viral Proteins