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Sci Rep. 2015 Oct 15;5:15403. doi: 10.1038/srep15403.

The HIV matrix protein p17 induces hepatic lipid accumulation via modulation of nuclear receptor transcriptoma.

Author information

1
Department of Surgical and Biomedical Sciences, Section of gastroenterology, University of Perugia, Perugia, Italy.
2
Department of Medicine, Section of Infectious diseases, University of Perugia, Perugia, Italy.
3
Department of Biomedical and Pharmaceutical Sciences, University of Salerno, Fisciano, Italy.
4
Department of Experimental Medicine and Biochemical Sciences, Section of Anatomic Pathology and Histology, University of Perugia, Perugia, Italy.
5
Hospital S.M. Misericordia, Perugia, Italy.

Abstract

Liver disease is the second most common cause of mortality in HIV-infected persons. Exactly how HIV infection per se affects liver disease progression is unknown. Here we have investigated mRNA expression of 49 nuclear hormone receptors (NRs) and 35 transcriptional coregulators in HepG2 cells upon stimulation with the HIV matrix protein p17. This viral protein regulated mRNA expression of some NRs among which LXRα and its transcriptional co-activator MED1 were highly induced at mRNA level. Dissection of p17 downstream intracellular pathway demonstrated that p17 mediated activation of Jak/STAT signaling is responsible for the promoter dependent activation of LXR. The treatment of both HepG2 as well as primary hepatocytes with HIV p17 results in the transcriptional activation of LXR target genes (SREBP1c and FAS) and lipid accumulation. These effects are lost in HepG2 cells pre-incubated with a serum from HIV positive person who underwent a vaccination with a p17 peptide as well as in HepG2 cells pre-incubated with the natural LXR antagonist gymnestrogenin. These results suggest that HIV p17 affects NRs and their related signal transduction thus contributing to the progression of liver disease in HIV infected patients.

PMID:
26469385
PMCID:
PMC4606811
DOI:
10.1038/srep15403
[Indexed for MEDLINE]
Free PMC Article

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