Down-regulation of 5S rRNA by miR-150 and miR-383 enhances c-Myc-rpL11 interaction and inhibits proliferation of esophageal squamous carcinoma cells

Xinyu Wang; Yanli Ren; Zhiqiong Wang; Xiangyu Xiong; Sichong Han; Wenting Pan; Hongwei Chen; Liqing Zhou; Changchun Zhou; Qipeng Yuan; Ming Yang

doi:10.1016/j.febslet.2015.11.012

Down-regulation of 5S rRNA by miR-150 and miR-383 enhances c-Myc-rpL11 interaction and inhibits proliferation of esophageal squamous carcinoma cells

FEBS Lett. 2015 Dec 21;589(24 Pt B):3989-97. doi: 10.1016/j.febslet.2015.11.012. Epub 2015 Nov 24.

Authors

Affiliations

¹ State Key Laboratory of Chemical Resource Engineering, Beijing Laboratory of Biomedical Materials, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China; Department of Genetics, Harvard Medical School, Boston, MA, USA.
² State Key Laboratory of Chemical Resource Engineering, Beijing Laboratory of Biomedical Materials, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China.
³ Department of Radiation Oncology, Huaian No. 2 Hospital, Huaian, Jiangsu Province, China.
⁴ Clinical Laboratory, Shandong Cancer Hospital, Shandong Academy of Medical Sciences, Jinan, Shandong Province, China.
⁵ State Key Laboratory of Chemical Resource Engineering, Beijing Laboratory of Biomedical Materials, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China. Electronic address: yangm@mail.buct.edu.cn.

PMID: 26606907
DOI: 10.1016/j.febslet.2015.11.012

Abstract

5S rRNA plays an important part in ribosome biology and is over-expression in multiple cancers. In this study, we found that 5S rRNA is a direct target of miR-150 and miR-383 in esophageal squamous cell carcinoma (ESCC). Overexpression of miR-150 and miR-383 inhibited ESCC cell proliferation in vitro and in vivo. Moreover, 5S rRNA silencing by miR-150 and miR-383 might intensify rpL11-c-Myc interaction, which attenuated role of c-Myc as an oncogenic transcriptional factor and dysregulation of multiple c-Myc target genes. Taken together, our results highlight the involvement of miRNAs in ribosomal regulation during tumorigenesis.

Keywords: 5S rRNA; Esophageal squamous cell carcinoma; c-Myc; miR-150; miR-383.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Carcinogenesis
Carcinoma, Squamous Cell / metabolism*
Carcinoma, Squamous Cell / pathology
Cell Line, Tumor
Cell Proliferation
Down-Regulation
Esophageal Neoplasms / metabolism*
Esophageal Neoplasms / pathology
Esophageal Squamous Cell Carcinoma
Female
Gene Expression Regulation, Neoplastic
Humans
Mice, Nude
MicroRNAs / metabolism*
Neoplasm Proteins / agonists
Neoplasm Proteins / metabolism
Neoplasm Transplantation
Proto-Oncogene Proteins c-myc / agonists*
Proto-Oncogene Proteins c-myc / metabolism
RNA / metabolism
RNA Interference*
RNA, Neoplasm / antagonists & inhibitors
RNA, Neoplasm / metabolism
RNA, Ribosomal, 5S / antagonists & inhibitors*
RNA, Ribosomal, 5S / metabolism
Ribosomal Proteins / agonists*
Ribosomal Proteins / metabolism
Tumor Burden

Substances

MIRN150 microRNA, human
MIRN383 microRNA, human
MYC protein, human
MicroRNAs
Neoplasm Proteins
Proto-Oncogene Proteins c-myc
RNA, Neoplasm
RNA, Ribosomal, 5S
RNA, recombinant
Ribosomal Proteins
ribosomal protein L11
RNA