A Highly Conserved Residue of the HIV-1 gp120 Inner Domain Is Important for Antibody-Dependent Cellular Cytotoxicity Responses Mediated by Anti-cluster A Antibodies

Shilei Ding; Maxime Veillette; Mathieu Coutu; Jérémie Prévost; Louise Scharf; Pamela J Bjorkman; Guido Ferrari; James E Robinson; Christina Stürzel; Beatrice H Hahn; Daniel Sauter; Frank Kirchhoff; George K Lewis; Marzena Pazgier; Andrés Finzi

doi:10.1128/JVI.02779-15

A Highly Conserved Residue of the HIV-1 gp120 Inner Domain Is Important for Antibody-Dependent Cellular Cytotoxicity Responses Mediated by Anti-cluster A Antibodies

J Virol. 2015 Dec 4;90(4):2127-34. doi: 10.1128/JVI.02779-15. Print 2016 Feb 15.

Authors

Affiliations

¹ Centre de Recherche du CHUM, Montreal, QC, Canada Department of Microbiology, Infectiology and Immunology, Université de Montréal, Montreal, QC, Canada.
² Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA.
³ Duke Human Vaccine Institute, Duke University Medical Center, Durham, North Carolina, USA.
⁴ Department of Pediatrics, Tulane University School of Medicine, New Orleans, Louisiana, USA.
⁵ Institute of Molecular Virology, Ulm University Medical Center, Ulm, Germany.
⁶ Department of Medicine and Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
⁷ Institute of Human Virology and Department of Biochemistry and Molecular Biology of University of Maryland School of Medicine, Baltimore, Maryland, USA.
⁸ Centre de Recherche du CHUM, Montreal, QC, Canada Department of Microbiology, Infectiology and Immunology, Université de Montréal, Montreal, QC, Canada Department of Microbiology and Immunology, McGill University, Montreal, QC, Canada andres.finzi@umontreal.ca.

Abstract

Previous studies have shown that sera from HIV-1-infected individuals contain antibodies able to mediate antibody-dependent cellular cytotoxicity (ADCC). These antibodies preferentially recognize envelope glycoprotein (Env) epitopes induced upon CD4 binding. Here, we show that a highly conserved tryptophan at position 69 of the gp120 inner domain is important for ADCC mediated by anti-cluster A antibodies and sera from HIV-1-infected individuals.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Antibody-Dependent Cell Cytotoxicity*
Conserved Sequence
HIV Antibodies / immunology*
HIV Envelope Protein gp120 / immunology*
HIV-1 / immunology*
Humans

Substances

HIV Antibodies
HIV Envelope Protein gp120
gp120 protein, Human immunodeficiency virus 1

Abstract

Publication types

MeSH terms

Substances

Grants and funding