Genome sequences of two closely related strains of Escherichia coli K-12 GM4792

Yan-Cong Zhang; Yan Zhang; Bi-Ru Zhu; Bo-Wen Zhang; Chuan Ni; Da-Yong Zhang; Ying Huang; Erli Pang; Kui Lin

doi:10.1186/s40793-015-0114-x

Genome sequences of two closely related strains of Escherichia coli K-12 GM4792

Stand Genomic Sci. 2015 Dec 10:10:125. doi: 10.1186/s40793-015-0114-x. eCollection 2015.

Authors

Yan-Cong Zhang¹, Yan Zhang², Bi-Ru Zhu¹, Bo-Wen Zhang¹, Chuan Ni³, Da-Yong Zhang¹, Ying Huang⁴, Erli Pang¹, Kui Lin¹

Affiliations

¹ State Key Laboratory of Earth Surface Processes and Resource Ecology and MOE Key Laboratory for Biodiversity Science and Ecological Engineering, College of Life Sciences, Beijing Normal University, 19 Xinjiekouwai Street, Beijing, 100875 China.
² State Key Laboratory of Earth Surface Processes and Resource Ecology and MOE Key Laboratory for Biodiversity Science and Ecological Engineering, College of Life Sciences, Beijing Normal University, 19 Xinjiekouwai Street, Beijing, 100875 China ; Present address: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101 China.
³ State Key Laboratory of Earth Surface Processes and Resource Ecology and MOE Key Laboratory for Biodiversity Science and Ecological Engineering, College of Life Sciences, Beijing Normal University, 19 Xinjiekouwai Street, Beijing, 100875 China ; Present address: The second high school attached to Beijing Normal University, Beijing, 100192 China.
⁴ State Key Laboratory for Infectious Disease Prevention and Control, and National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206 China.

Abstract

Escherichia coli lab strains K-12 GM4792 Lac(+) and GM4792 Lac(-) carry opposite lactose markers, which are useful for distinguishing evolved lines as they produce different colored colonies. The two closely related strains are chosen as ancestors for our ongoing studies of experimental evolution. Here, we describe the genome sequences, annotation, and features of GM4792 Lac(+) and GM4792 Lac(-). GM4792 Lac(+) has a 4,622,342-bp long chromosome with 4,061 protein-coding genes and 83 RNA genes. Similarly, the genome of GM4792 Lac(-) consists of a 4,621,656-bp chromosome containing 4,043 protein-coding genes and 74 RNA genes. Genome comparison analysis reveals that the differences between GM4792 Lac(+) and GM4792 Lac(-) are minimal and limited to only the targeted lac region. Moreover, a previous study on competitive experimentation indicates the two strains are identical or nearly identical in survivability except for lactose utilization in a nitrogen-limited environment. Therefore, at both a genetic and a phenotypic level, GM4792 Lac(+) and GM4792 Lac(-), with opposite neutral markers, are ideal systems for future experimental evolution studies.

Keywords: Escherichia coli K12; Experimental evolution; GM4792; Genome comparison; Gram-negative; Lactose; Variant analysis.

Publication types

Case Reports