Hepatitis B Virus Infection of a Mouse Hepatic Cell Line Reconstituted with Human Sodium Taurocholate Cotransporting Polypeptide

J Virol. 2016 Apr 14;90(9):4827-4831. doi: 10.1128/JVI.02832-15. Print 2016 May.

Abstract

Hepatitis B virus (HBV) enters hepatocytes via its receptor, human sodium taurocholate cotransporting polypeptide (hNTCP). So far, HBV infection has been achieved only in human hepatic cells reconstituted with hNTCP and not in cells of mouse origin. Here, the first mouse liver cell line (AML12) which gains susceptibility to HBV upon hNTCP expression is described. Thus, HBV infection of receptor-expressing mouse hepatocytes does not principally require a human cofactor but can be triggered by endogenous murine determinants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Disease Susceptibility
  • Gene Expression
  • Hepatitis B / genetics
  • Hepatitis B / metabolism*
  • Hepatitis B / virology*
  • Hepatitis B virus / physiology*
  • Hepatitis Delta Virus / physiology
  • Hepatocytes / metabolism
  • Hepatocytes / virology
  • Host-Pathogen Interactions
  • Humans
  • Mice
  • Organic Anion Transporters, Sodium-Dependent / genetics
  • Organic Anion Transporters, Sodium-Dependent / metabolism*
  • Symporters / genetics
  • Symporters / metabolism*
  • Virus Internalization

Substances

  • Organic Anion Transporters, Sodium-Dependent
  • Symporters
  • sodium-bile acid cotransporter