Mutation of the key residue for extraribosomal function of ribosomal protein S19 cause increased grooming behaviors in mice

Neurosci Lett. 2016 Aug 26:629:221-226. doi: 10.1016/j.neulet.2016.07.022. Epub 2016 Jul 15.

Abstract

Ribosomal protein S19 (RP S19) possesses ribosomal function as RP S19 monomer and extraribosomal function as cross-linked RP S19 oligomers which function as a ligand of the complement 5a (C5a) receptor (CD88). We have generated a Gln137Glu-RP S19 knock-in (KI) mouse, which is shown to possess the weakened extraribosomal function of RP S19. Because whether the extraribosomal function of RP S19 has a role in brain function had been unclear, we performed behavioral analysis on these mice and demonstrated that KI mice displayed an increased grooming behavior during open-field test and elevated plus maze test and an enhanced freezing behavior in contextual fear conditioning test. These results suggest an involvement of RP S19 oligomers in some anxiety-like behavior, especially grooming behavior.

Keywords: Anxiety-like behavior; Complement system; Extraribosomal function; Fear memory; Grooming behavior; Ribosomal protein S19.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anxiety / genetics*
  • Anxiety / physiopathology*
  • Chemotaxis
  • Conditioning, Classical / physiology
  • Fear / physiology
  • Gene Knock-In Techniques
  • Grooming / physiology*
  • Male
  • Memory / physiology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Monocytes / physiology
  • Mutation*
  • Ribosomal Proteins / genetics*
  • Ribosomal Proteins / physiology*

Substances

  • Ribosomal Proteins
  • ribosomal protein S19