Identification of Circulating MiR-25 as a Potential Biomarker for Pancreatic Cancer Diagnosis

Cell Physiol Biochem. 2016;39(5):1716-1722. doi: 10.1159/000447872. Epub 2016 Sep 19.

Abstract

Background: The aim of this study was to identify novel microRNAs for potential use in the diagnosis of pancreatic cancer (PaC).

Methods: A total of 1063 serum samples from 303 patients with PaC were collected, and the expression level of miR-25 was measured using quantitative real-time PCR (qRT-PCR).

Results: We found that miR-25 had significant diagnostic value for the differential diagnosis of PaC in normal controls with an AUC (the area under the ROC curve) of 0.915 (95% CI: 0.893-0.937) that was significantly higher compared with an AUC of 0.725 for serum tumor marker carcinoembryonic antigen (CEA) and an AUC of 0.844 for CA19-9.

Conclusions: These data suggest that serum miR-25 has strong potential as a novel biomarker for the early detection of PaC.

MeSH terms

  • Adult
  • Aged
  • Area Under Curve
  • Biomarkers, Tumor / blood*
  • Biomarkers, Tumor / genetics
  • CA-19-9 Antigen / blood
  • CA-19-9 Antigen / genetics
  • Carcinoembryonic Antigen / blood
  • Carcinoembryonic Antigen / genetics
  • Case-Control Studies
  • Diagnosis, Differential
  • Early Diagnosis
  • Female
  • Gastrointestinal Neoplasms / blood
  • Gastrointestinal Neoplasms / diagnosis*
  • Gastrointestinal Neoplasms / genetics
  • Gastrointestinal Neoplasms / pathology
  • Gene Expression
  • Humans
  • Male
  • MicroRNAs / blood*
  • MicroRNAs / genetics
  • Middle Aged
  • Neoplasm Staging
  • Pancreas / metabolism
  • Pancreas / pathology
  • Pancreatic Neoplasms / blood
  • Pancreatic Neoplasms / diagnosis*
  • Pancreatic Neoplasms / genetics
  • Pancreatic Neoplasms / pathology
  • Pancreatitis, Chronic / blood
  • Pancreatitis, Chronic / diagnosis*
  • Pancreatitis, Chronic / genetics
  • Pancreatitis, Chronic / pathology
  • ROC Curve

Substances

  • Biomarkers, Tumor
  • CA-19-9 Antigen
  • Carcinoembryonic Antigen
  • MIRN25 microRNA, human
  • MicroRNAs