Surface phosphonation enhances hydroxyapatite coating adhesion on polyetheretherketone and its osseointegration potential

Acta Biomater. 2017 Jan 1:47:149-158. doi: 10.1016/j.actbio.2016.10.004. Epub 2016 Oct 4.

Abstract

Polyetheretherketone (PEEK) has excellent mechanical properties, biocompatibility, chemical resistance and radiolucency, making it suitable for use as orthopedic implants. However, its surface is hydrophobic and bioinert, and surface modification is required to improve its bioactivity. In this work, we showed that grafting phosphonate groups via diazonium chemistry enhances the bioactivity of PEEK. Decreased contact angle indicated reduced hydrophobicity as a result of the treatment and X-ray photoelectron spectroscopy (XPS) confirmed the attachment of phosphonate groups to the surface. The surface treatment not only accelerated hydroxyapatite (HA) deposition after immersion in simulated body fluid but also significantly increased the adhesion strength of HA particles on PEEK. MC3T3-E1 cell viability, metabolic activity and deposition of calcium-containing minerals were also enhanced by the phosphonation. After three months of implantation in a critical size calvarial defect model, a fibrous capsule surrounded untreated PEEK while no fibrous capsule was observed around the treated PEEK. Instead, mineral deposition was observed in the region between the treated PEEK implant and underlying bone. This work introduces a simple method to improve the potential of PEEK-based orthopedic implants.

Statement of significance: We have introduced phosphonate groups on the surface of PEEK substrates using diazonium chemistry. Our results show that the treatment not only increased the adhesion strength of hydroxyapatite particles deposited on PEEK in vitro by approximately 40% compared to unmodified PEEK, but also improved the metabolic activity and mineralization of MC3T3-E1 cells. When implanted in cranial defects in rats, the phosphonate coating enhanced the osseointegration of PEEK by successfully preventing the formation of a fibrous capsule and favoring mineral deposition between the implant and the surrounding bone. This work introduces a simple method to improve the potential of PEEK-based orthopedic implants, particularly those with complex shapes.

Keywords: Adhesion strength; Biomineralization; Diazonium chemistry; In vivo osseointegration; PEEK.

MeSH terms

  • Animals
  • Benzophenones
  • Body Fluids / chemistry
  • Cell Adhesion
  • Cell Line
  • Coated Materials, Biocompatible / chemistry*
  • Durapatite / chemistry*
  • Ketones / chemistry*
  • Mice
  • Osseointegration*
  • Phosphorous Acids / chemistry*
  • Polyethylene Glycols / chemistry*
  • Polymers
  • Rats, Sprague-Dawley
  • Spectroscopy, Fourier Transform Infrared
  • Surface Properties

Substances

  • Benzophenones
  • Coated Materials, Biocompatible
  • Ketones
  • Phosphorous Acids
  • Polymers
  • phosphonic acid
  • polyetheretherketone
  • Polyethylene Glycols
  • Durapatite