Single-Cell Multiomics: Multiple Measurements from Single Cells

Trends Genet. 2017 Feb;33(2):155-168. doi: 10.1016/j.tig.2016.12.003. Epub 2017 Jan 13.

Abstract

Single-cell sequencing provides information that is not confounded by genotypic or phenotypic heterogeneity of bulk samples. Sequencing of one molecular type (RNA, methylated DNA or open chromatin) in a single cell, furthermore, provides insights into the cell's phenotype and links to its genotype. Nevertheless, only by taking measurements of these phenotypes and genotypes from the same single cells can such inferences be made unambiguously. In this review, we survey the first experimental approaches that assay, in parallel, multiple molecular types from the same single cell, before considering the challenges and opportunities afforded by these and future technologies.

Keywords: epigenomics; genomics; multiomics; proteomics; single cell; transcriptomics.

Publication types

  • Review
  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Methylation / genetics*
  • High-Throughput Nucleotide Sequencing
  • Humans
  • Metabolome / genetics*
  • Proteome / genetics*
  • Single-Cell Analysis / methods
  • Transcriptome / genetics*

Substances

  • Proteome