The Scale of Positive and Negative Experience (SPANE) measures the frequency of positive and negative affect. It consists of two subscales, one for positive and one for negative affect with six adjectives each and assesses a broad range of emotions. In this study, the psychometric properties of the German version of the scale were explored with reliability and confirmatory factorial analysis by using a German sample of N = 498. To examine the convergent validity of the SPANE we correlated its subscales with other well-being and happiness measures. Retest-reliabilities were tested after four weeks, taking into account the occurrence of emotionally significant life events. The scale was also applied to evaluate a training of subjective well-being and in a study on text comprehensibility. The results confirmed the postulated two-factor structure of the SPANE and showed good psychometric properties and convergent validity. In conclusion, the German version of the scale behaves in accordance with the original scale and may be used in future studies of well-being.