Not5-dependent co-translational assembly of Ada2 and Spt20 is essential for functional integrity of SAGA

Sari Kassem; Zoltan Villanyi; Martine A Collart

doi:10.1093/nar/gkw1059

Not5-dependent co-translational assembly of Ada2 and Spt20 is essential for functional integrity of SAGA

Nucleic Acids Res. 2017 Feb 17;45(3):1186-1199. doi: 10.1093/nar/gkw1059.

Authors

Sari Kassem¹, Zoltan Villanyi¹, Martine A Collart¹

Affiliation

¹ Department of Microbiology and Molecular Medicine, Faculty of Medicine, Institute of Genetics and Genomics Geneva, University of Geneva, Geneva, Switzerland

Abstract

Acetylation of histones regulates gene expression in eukaryotes. In the yeast Saccharomyces cerevisiae it depends mainly upon the ADA and SAGA histone acetyltransferase complexes for which Gcn5 is the catalytic subunit. Previous screens have determined that global acetylation is reduced in cells lacking subunits of the Ccr4–Not complex, a global regulator of eukaryotic gene expression. In this study we have characterized the functional connection between the Ccr4–Not complex and SAGA. We show that SAGA mRNAs encoding a core set of SAGA subunits are tethered together for co-translational assembly of the encoded proteins. Ccr4–Not subunits bind SAGA mRNAs and promote the co-translational assembly of these subunits. This is needed for integrity of SAGA. In addition, we determine that a glycolytic enzyme, the glyceraldehyde-3-phosphate dehydrogenase Tdh3, a prototypical moonlighting protein, is tethered at this site of Ccr4–Not-dependent co-translational SAGA assembly and functions as a chaperone.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gene Expression Regulation, Fungal
Genes, Fungal
Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / chemistry
Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / genetics
Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / metabolism
Histone Acetyltransferases / chemistry
Histone Acetyltransferases / genetics
Histone Acetyltransferases / metabolism
Models, Biological
Mutation
Protein Multimerization / genetics
Protein Subunits
RNA, Fungal / genetics
RNA, Fungal / metabolism
RNA, Messenger / genetics
RNA, Messenger / metabolism
Ribonucleases / chemistry
Ribonucleases / genetics
Ribonucleases / metabolism
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / metabolism
Saccharomyces cerevisiae Proteins / chemistry
Saccharomyces cerevisiae Proteins / genetics*
Saccharomyces cerevisiae Proteins / metabolism*
Trans-Activators / chemistry
Trans-Activators / genetics*
Trans-Activators / metabolism*
Transcription Factors / chemistry
Transcription Factors / genetics*
Transcription Factors / metabolism*

Substances

ADA2 protein, S cerevisiae
NOT5 protein, S cerevisiae
Protein Subunits
RNA, Fungal
RNA, Messenger
SAGA complex, S cerevisiae
SPT20 protein, S cerevisiae
Saccharomyces cerevisiae Proteins
Trans-Activators
Transcription Factors
Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)
TDH3 protein, S cerevisiae
GCN5 protein, S cerevisiae
Histone Acetyltransferases
CCR4 protein, S cerevisiae
Ribonucleases